In the early s, Roger Penrose introduced new mathematical techniques to solve Einstein's equations where exact answers were unavailable because of asymmetry.
During this lab, we were to use fruit flies to do genetic crosses, learn how to determine the sex of fruit flies and recognize contrasting phenotypes, and collect data from F1 and F2 generations and analyze the results of a monohybrid, dihybrid, or sex-linked cross.
To determine the sex of fruit flies and recognize contrasting phenotypes Activity Awe took a vial flies and put them to sleep with FlyNap. Once the flies were asleep, we were able to sort out some flies for observation. We referred to Figure 2, which is a figure of a female and male fruit fly and points out the differences between the two.
Females have no sex comb on their front legs and they have an abdomen with several stripes.
Males have sex combs on their legs and have a heavily pigmented tip on the end of their abdomens. We were able to determine the sex of the flies and view wild phenotypes and mutant phenotypes. We recorded our data and observations in Table 1 and Table 2.
The outcome was the phenotypes for wild and mutant types. I liked this activity because we were able to put flies to sleep on our own and be able to distinguish between sex, antennas, eyes, and wings.
After putting the flies to sleep, we opened the vial and sorted out 5 females and 5 males. We recorded the amounts and their phenotype in Table 2.
While keeping the 10 flies in a petri dish, we made a vial with instant Drosophila Medium and one cup of water. Once it solidified, we sprinkled yeast on top of the mixture. We then put some cotton netting in the vial for the flies to rest on instead of the medium. We added the flies to the vial and closed it.
To remove F1 adults from vials Activity Cwe needed to separate the parents from the offspring by putting them in separate vials.
After we tried as much as we could to separate the parents of the F1 generation, we put the parents in the morgue. To score the phenotypes of the F2 flies Activity Dwe put the adult F2 flies to sleep with FlynNap and put them in a petri dish. We observed the sex and phenotypes of the offspring and recorded the observations in Table 3.
After observing and recording the sex and phenotypes of all the flies collected, we place them in the fly morgue. For Activity E, we did a data analysis by using the chi-squared test.
We took the data from the F1 generation and F2 generation to use the chi-squared test on. Then we used the degrees of freedom to see if the answer is significant.
I liked this activity because we were able to use make to determine if the data is significant or not.AP Biology Genetics of Drosophila Lab Report. AP Biology Transformation Lab Report. Mendelian Genetics Lab.
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Transcript of Drosophila Lab. Genetics of Fruit Flies Linkages (part 3) The third cross was a Female wild type and a Male (black body, curved wings). After completing the Drosophila lab, we concluded that the star-eyed trait is dominant to wild type flies by doing multiple crosses.
We also concluded that the genotype AA.